Influenza A virus transfectants with chimeric hemagglutinins containing epitopes from different subtypes.
Identifieur interne : 001F27 ( Main/Exploration ); précédent : 001F26; suivant : 001F28Influenza A virus transfectants with chimeric hemagglutinins containing epitopes from different subtypes.
Auteurs : S Q Li [États-Unis] ; J L Schulman ; T. Moran ; C. Bona ; P. PaleseSource :
- Journal of virology [ 0022-538X ] ; 1992.
Descripteurs français
- KwdFr :
- ADN viral, Animaux, Anticorps antiviraux (immunologie), Chimère, Données de séquences moléculaires, Glycoprotéine hémagglutinine du virus influenza, Hémagglutinines virales (génétique), Hémagglutinines virales (immunologie), Lignée cellulaire, Mutagenèse dirigée, Souris, Souris de lignée BALB C, Séquence d'acides aminés, Séquence nucléotidique, Transfection, Virus de la grippe A (génétique), Virus de la grippe A (immunologie), Épitopes (génétique), Épitopes (immunologie).
- MESH :
- génétique : Hémagglutinines virales, Virus de la grippe A, Épitopes.
- immunologie : Anticorps antiviraux, Hémagglutinines virales, Virus de la grippe A, Épitopes.
- ADN viral, Animaux, Chimère, Données de séquences moléculaires, Glycoprotéine hémagglutinine du virus influenza, Lignée cellulaire, Mutagenèse dirigée, Souris, Souris de lignée BALB C, Séquence d'acides aminés, Séquence nucléotidique, Transfection.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (immunology), Base Sequence, Cell Line, Chimera, DNA, Viral, Epitopes (genetics), Epitopes (immunology), Hemagglutinin Glycoproteins, Influenza Virus, Hemagglutinins, Viral (genetics), Hemagglutinins, Viral (immunology), Influenza A virus (genetics), Influenza A virus (immunology), Mice, Mice, Inbred BALB C, Molecular Sequence Data, Mutagenesis, Site-Directed, Transfection.
- MESH :
- chemical , genetics : Epitopes, Hemagglutinins, Viral.
- chemical , immunology : Antibodies, Viral, Epitopes, Hemagglutinins, Viral.
- genetics : Influenza A virus.
- immunology : Influenza A virus.
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Chimera, DNA, Viral, Hemagglutinin Glycoproteins, Influenza Virus, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Mutagenesis, Site-Directed, Transfection.
Abstract
Influenza virus transfectants with chimeric hemagglutinins were constructed by using a ribonucleoprotein transfection method. Transfectants W(H1)-H2 and W(H1)-H3 contained A/WSN/33(H1N1) (WSN) hemagglutinins in which the six-amino-acid loop (contained in antigenic site B) was replaced by the corresponding structures of influenza viruses A/Japan/57(H2N2) and A/Hong Kong/8/68(H3N2) (HK), respectively. Serological analysis indicated that the W(H1)-H3 transfectant virus reacted with antibodies against both the WSN and HK viruses in hemagglutination inhibition and plaque neutralization assays. Furthermore, mice immunized with W(H1)-H3 transfectant virus produced antibodies to the WSN and HK viruses. The results demonstrate that influenza virus transfectants can be engineered to express epitopes of different subtypes on their hemagglutinins.
PubMed: 1370088
Affiliations:
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Le document en format XML
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Sinai School of Medicine, New York, New York 10029.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Microbiology, Mt. Sinai School of Medicine, New York</wicri:cityArea></affiliation></author><author><name sortKey="Schulman, J L" sort="Schulman, J L" uniqKey="Schulman J" first="J L" last="Schulman">J L Schulman</name></author><author><name sortKey="Moran, T" sort="Moran, T" uniqKey="Moran T" first="T" last="Moran">T. Moran</name></author><author><name sortKey="Bona, C" sort="Bona, C" uniqKey="Bona C" first="C" last="Bona">C. Bona</name></author><author><name sortKey="Palese, P" sort="Palese, P" uniqKey="Palese P" first="P" last="Palese">P. Palese</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Viral (immunology)</term><term>Base Sequence</term><term>Cell Line</term><term>Chimera</term><term>DNA, Viral</term><term>Epitopes (genetics)</term><term>Epitopes (immunology)</term><term>Hemagglutinin Glycoproteins, Influenza Virus</term><term>Hemagglutinins, Viral (genetics)</term><term>Hemagglutinins, Viral (immunology)</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Transfection</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral</term><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Chimère</term><term>Données de séquences moléculaires</term><term>Glycoprotéine hémagglutinine du virus influenza</term><term>Hémagglutinines virales (génétique)</term><term>Hémagglutinines virales (immunologie)</term><term>Lignée cellulaire</term><term>Mutagenèse dirigée</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Transfection</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (immunologie)</term><term>Épitopes (génétique)</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Epitopes</term><term>Hemagglutinins, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Epitopes</term><term>Hemagglutinins, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Hémagglutinines virales</term><term>Virus de la grippe A</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Hémagglutinines virales</term><term>Virus de la grippe A</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cell Line</term><term>Chimera</term><term>DNA, Viral</term><term>Hemagglutinin Glycoproteins, Influenza Virus</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN viral</term><term>Animaux</term><term>Chimère</term><term>Données de séquences moléculaires</term><term>Glycoprotéine hémagglutinine du virus influenza</term><term>Lignée cellulaire</term><term>Mutagenèse dirigée</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Influenza virus transfectants with chimeric hemagglutinins were constructed by using a ribonucleoprotein transfection method. Transfectants W(H1)-H2 and W(H1)-H3 contained A/WSN/33(H1N1) (WSN) hemagglutinins in which the six-amino-acid loop (contained in antigenic site B) was replaced by the corresponding structures of influenza viruses A/Japan/57(H2N2) and A/Hong Kong/8/68(H3N2) (HK), respectively. Serological analysis indicated that the W(H1)-H3 transfectant virus reacted with antibodies against both the WSN and HK viruses in hemagglutination inhibition and plaque neutralization assays. Furthermore, mice immunized with W(H1)-H3 transfectant virus produced antibodies to the WSN and HK viruses. The results demonstrate that influenza virus transfectants can be engineered to express epitopes of different subtypes on their hemagglutinins.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><noCountry><name sortKey="Bona, C" sort="Bona, C" uniqKey="Bona C" first="C" last="Bona">C. Bona</name><name sortKey="Moran, T" sort="Moran, T" uniqKey="Moran T" first="T" last="Moran">T. Moran</name><name sortKey="Palese, P" sort="Palese, P" uniqKey="Palese P" first="P" last="Palese">P. Palese</name><name sortKey="Schulman, J L" sort="Schulman, J L" uniqKey="Schulman J" first="J L" last="Schulman">J L Schulman</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Li, S Q" sort="Li, S Q" uniqKey="Li S" first="S Q" last="Li">S Q Li</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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